PUBLICATIONS

Structural and functional diversity in plant specialized metabolism signals and products : the case of oxylipins and triterpenes  2023
Elia Lacchini, Jhon Venegas-Molina, Alain Goossens
Current Opinion in Plant Biology
https://linkinghub.elsevier.com/retrieve/pii/S1369-5266(23)00036-5

Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus  2023
Charlotte De Bruyn, Tom Ruttink, Elia Lacchini, Stephane Rombauts, Annelies Haegeman, Ellen De Keyser, Christof Van Poucke, Sandrien Desmet, Thomas B Jacobs, Tom Eeckhaut, Alain Goossens, Katrijn Van Laere
Frontiers in Plant Science
https://www.frontiersin.org/articles/10.3389/fpls.2023.1200253/full

The saponin bomb: a nucleolar‐localized β‐glucosidase hydrolyzes triterpene saponins in <i>Medicago truncatula  2023
Elia Lacchini; Marie‐Laure Erffelinck; Jan Mertens; Shirley Marcou; Francisco Javier Molina‐Hidalgo; Oren Tzfadia; Jhon Venegas‐Molina; Pablo D. Cárdenas; Jacob Pollier; Aldo Tava; Søren Bak; Monica Höfte; Alain Goossens
New Phytologist
https://biblio.ugent.be/publication/01GQPCKQQEM8577KV3VPYJSD69

Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering : achievements and perspectives  2023
Sara Selma; Evi Ceulemans; Alain Goossens; Elia Lacchini
Current Opinion in Biotechnology
https://biblio.ugent.be/publication/01GKV0106VQ8WRS1YWK30JAK51

Suicide nanoplasmids coding for ribosome-inactivating proteins  2022
Hardy Mitdank, Meike Tröger, Alexander Sonntag, Nima Amini Shirazi, Eric Woith, Hendrik Fuchs, Dennis Kobelt, Wolfgang Walther, Alexander Weng
European Journal of Pharmaceutical Sciences
https://www.sciencedirect.com/science/article/pii/S0928098721004085

Within and beyond organelle engineering: strategies for increased terpene production in yeasts and plants  2022
Ana Cristina Jaramillo-Madrid; Elia Lacchini; Alain Goossens
Current Opinion in Green and Sustainable Chemistry
https://biblio.ugent.be/publication/8734977

Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula  2022
Bianca Ribeiro, Marie-Laure Erffelinck, Elia Lacchini, Evi Ceulemans, Maite Colinas, Clara Williams, Evelien Van Hamme, Rebecca De Clercq, Maria Perassolo, Alain Goossens
Frontiers in Plant Science
https://www.frontiersin.org/articles/10.3389/fpls.2022.903793/full

Jasmonate: A hormone of primary importance for plant metabolism  2022
Elia Lacchini; Alain Goossens; Hieu Nguyen Trang
Current Opinion in Plant Biology
https://biblio.ugent.be/publication/8743927

An advanced method for the small-scale production of high-quality minicircle DNA  2021
Hardy Mitdank, Simko Sama, Meike Tröger, Maria Francesca Testa, Mattia Ferrarese, Dario Balestra, Mirko Pinotti, Alexander Weng
International Journal of Pharmaceutics
https://doi.org/10.1016/j.ijpharm.2021.120830

A Seed-SpecificRegulator of Triterpene Saponin Biosynthesis in Medicago truncatula  2020
Bianca Ribeiro, Elia Lacchini, Keylla U Bicalho, Jan Mertens, Philipp Arendt, Robin Vanden Bossche, Gabriela Calegario, Lore Gryffroy, Evi Ceulemans, Julia Buitink, Alain Goossens, Jacob Pollier
Plant Cell
https://academic.oup.com/plcell/article/32/6/2020/6115680

Combinatorial Control of Plant Specialized Metabolism: Mechanisms, Functions, and Consequences  2020
Elia Lacchini, Alain Goossens
Annual Review of Cell and Developmental Biology
https://www.annualreviews.org/doi/10.1146/annurev-cellbio-011620-031429?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed


PUBLISHABLE SUMMARIES

ENDOSCAPE final publishable Summary at project end (Mo 54) - soon here

ENDOSCAPE Project Second Periodic Progress Report - Publishable Summary: progress in months 19-36

ENDOSCAPE Project First Period Progress Report - Publishable Summary: progress since the start of the project and the first 18 months

Project results can be found here: ENDOSCAPE @ EC Cordis 

International ENDOSCAPE Symposium

Venue: Berlin
Date: 08.-09.09.2022

Harnack-Haus, Tagungsstätte der Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
Ihnestraße 16-20, 14195 Berlin

Day 1 | 11:30 – 19:45

o Session 1: Gene Therapy – Where We Stand Today

• Non-viral gene therapy at a glance - Kazunori Kataoka, Tokyo
• Gene therapy in monogenetic diseases - Company presentation
• Suicide genes in cancer therapy - Company presentation
• Participant presentations

o Session 2: Endosomal Escape – The Strategies

• Strategies for intracellular delivery and endosomal escape - Vladimir P. Torchilin, Boston
• Photochemically-induced release - Pål Kristian Selbo, Oslo
• Intracellular drug delivery - Company presentation
• Glycosylated triterpenoids as endosomal escape enhancers - ENDOSCAPE-Partner FUB
• Cell penetrating peptides - Jean-Philippe Pellois, Texas

o Poster session

Day 2 | 08:15 – 18:30

o Session 3: Carriers of Endosomal Escape Enhancers and Genes

• Design of efficient intracellular delivery vehicles - Jan van Hest, Eindhoven
• Scaffolds to carry endosomal escape enhancers and targeting ligands, and to polyplex effector genes: PAMAMs, peptides and DNA - ENDOSCAPE-Partners CHARITE, FUB, UNITOV, USC
• Nanocarriers - Company presentation
• Chemical evolution refining intercellular mRNA, protein and Cas9/sgRNA delivery - Ernst Wagner, München

o Session 4: Endosomal Escape – What Happens?

• Intracellular routing of targeted payloads - Inge Zuhorn, Groningen
• Mechanisms of endosomal trafficking and escape by SO1861 - ENDOSCAPE-Partner MPG
• Efficacy of endosomal escape enhancers in vitro and ex vivo - ENDOSCAPE-Partner UNIFE, SAPREME, CHARITE
• Endosomal escape by photochemical internalization - Company presentation
• Participant presentation

o Poster session

o Session 5: GMP Production and Market

• Controlled production of endosomal escape enhancers in plants - ENDOSCAPE-Partners Fraunhofer and EXSY
• Gene discovery and synthetic biology for biosynthesis of endosomal escape enhancers in engineered plant cultures - ENDOSCAPE Partner VIB
• Gene therapy from bench to bedside - Company presentation
• Innovative solutions for GMP production of biopharmaceuticals - Company presentation
• Cost-of-goods analysis of the ENDOSCAPE technology - ENDOSCAPE-Partner UNIBAS
• Cost-effectiveness analysis of potential gene therapies for Hemophilia B based on ENDOSCAPE - ENDOSCAPE-Partner UNIBAS
• Participant presentations

o Session 6: Gene Therapy – Clinical Applications and Future Perspectives

• Gene-based nanomedicines for cancer therapy and brain delivery - Christine Dufès, Glasgow
• Transplantation of genetically modified hematopoietic stem cells - Fulvio Mavilio, Modena
• Gene therapy of Hemophilia - Antonia Follenzi, Novara
• Current status of non-viral gene therapy: The ENDOSCAPE symposium at a glance - ENDOSCAPE-Partners

Company presentations depending on registrations. If applicable, replacement by selected poster contributions as short presentations.

ENDOSCAPE partner presentations:
CHARITE: Charité – Universitätsmedizin Berlin
FUB: Freie Universität Berlin
UNITOV: The University of Rome Tor Vergata
USC: Universidade de Santiago de Compostela
MPG: Max-Planck-Gesellschaft zur Förderung der Wissenschaften
UNIFE: The Universita degli Studi di Ferrara
SAPREME: Sapreme Technologies B.V.
VIB: Vlaams Instituut Biotechnologie
Fraunhofer: Fraunhofer - Gesellschaft zur Förderung der Angewandten Forschung e.V.
EXSY: EXTRASYNTHESE SAS
UNIBAS: Universität Basel